Coding

Part:BBa_K1705004:Design

Designed by: Nathan Hunt   Group: iGEM15_MIT   (2015-09-17)


MoClo relE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 302


Design Notes

After taking the amino acid sequence for relE from E. coli, we codon optimized it for expression in Cytophaga hutchinsonii using [http://www.jcat.de JCat]. In order to make it more easy to synthesize (i.e. to increase G-C content in some regions), we also modified the sequence by hand using a table of codon frequencies in C. hutchinsonii from [http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=269798&aa=15&style=N Kazusa].

Source

Amino Acid Sequence Source: E. coli

References